Seamlessly rewriting the lab cloning manual
The YouTube video opens on a researcher studying an agarose gel on a UV light box. He slumps in resignation—clearly, his clones are a bust. He slowly shuffles over to the whiteboard, drops to his knees, and notches yet another failure under the heading “Assembly Attempts.” Tally total: 33. Anyone who has tried his or her hand at DNA cloning can relate. So simple in theory, yet persnickety in practice, cloning causes many headaches and sleepless nights. Cryptic restriction sites, unexpected fragment toxicity, phases of the moon—all seemingly play a hand… read more at BioTechniques.com (PDF)
Biotechnically Speaking 